培养环境条件对猕猴桃花粉萌发的影响 猕猴桃花粉萌发对授粉受精起着关键作用

培养环境条件对猕猴桃花粉萌发的影响 猕猴桃花粉教你如何在网上轻松赚钱萌发对授粉受精起着关键作用

教你如何在网上轻松赚钱QIXIUJUAN1,2,ZHANGSHAOLING1,FANGJINBAO2※

教你如何在网上轻松赚钱(1NANJINGNONGYEDAXUEYUANYIXUEYUAN,NANJING 210095;2ZHONGGUONONGYEKEXUEYUANZHENGZHOUGUOSHUYANJIUSUO,GUOSHUSHENGZHANGFAYUYUPINZHIKONGZHIZHONGDIANKAIFANGSHIYANSHI ZHENGZHOU 450009)

摘要:采用离体培养法,在筛选出最佳培养基后,研究猕猴桃花粉在不同培养时间、pH值、温度条件下花粉萌发和花粉管生长状况。结果表明:猕猴桃花粉离体萌发及花粉管生长的最适培养基为10%蔗糖+100mg/L硼酸+10mg/L Ca(NO3)2;离体培养最适观察时间为5.0h,最适培养温度为30℃,最佳培养pH值为6.0。

教你如何在网上轻松赚钱GUANJIANCI:MIHOUTAO;PEIYANGTIAOJIAN;HUAFENMENGFALV;HUAFENGUANSHENZHANG

Effect of Environment on Pollen Germination of Kiwi

Qi Xiu-juan1,2,ZHANG Shao-ling1,FANG Jin-bao2

教你如何在网上轻松赚钱(1Nanjing Agricultural University,Nanjing 210095;2Key Laboratory for Fruit Tree Growth, Development and Quality Control, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009)

Abstract:

The paper reports factors influencing pollen germination and tube growth of kiwi in vitro.With this one, the effects of different time, pH values, different storage temperatures and many amines were tested. The results showed that the most suitable culture medium for pollen germination and tube growth was 10% sugar+100mg/L boric acid+10mg/L Ca(NO3)2.The suitable watching time was after 5 hours, the best temperature for pollen germination and growth was 30℃ and the suitable pH value of culture medium was 6.0.

Key words:Kiwifruit;Effect of Environment; Pollen germination;Pollen tube growth

猕猴桃是雌雄异株的重要果树树种,生产中需要严格配置授粉树,但有时因花期不遇、恶劣天气或授粉昆虫数量的不足,导致授粉受精不良,而授粉受精是其丰产的基础,所以仅依靠天然授粉猕猴桃很难获得优质高产,故采用人工辅助授粉显得尤为必要。花粉萌发对授粉受精起着关键作用【1】,花粉萌发的好坏,除与花粉本身的质量有关外,与花粉萌发的环境条件和贮藏条件有密切关系[2]。关于猕猴桃人工辅助授粉的采粉时期、花粉贮藏条件以及Ca(NO3)2、Na6Mo7O24、MnSO4、NaCl、蔗糖、硼酸等因子对猕猴桃花粉的影响前人已做过一些研究[3,4,5],但花粉离体萌发最佳环境条件的研究则尚未见报道。本试验以美味猕猴桃花粉为研究材料,探讨其花粉的最佳离体培养环境条件,其结果可为猕猴桃生产上人工辅助授粉提供理论依据。

1.CAILIAOHEFANGFA

1.1CAILIAO:

教你如何在网上轻松赚钱CAILIAOYU2009NIAN5YUECHUXUANYONGZHONGGUONONGYEKEXUEYUANZHENGZHOUGUOSHUYANJIUSUOMIHOUTAOZIYUANPUNEITONG

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1.2HUAFENPEIYANGFA:

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1.3SHIYANCHULI

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1.3.4ZUISHIPEIYANGpHZHICEDING:CAIYONG‘ZHENGXIONG1HAO’HUAFENJINXINGGAISHIYAN。YINGYONGSHIYANSHAIXUANCHUDEZUIJIAPEIYANGJI(DI6ZHONGPEIYANGJI, QIZUFENJIANBIAO1)WEIJIBENPEIYANGJI,SHEDINGPEIYANGWENDU26℃,SHEZHIPEIYANGpHZHI(4.0、5.0、6.0、6.5、7.0、8.0、9.0)JINXINGPEIYANG,5 hHOUQUCHUCEQIMENGFALVJIHUAFENGUANZHANGDU。

2.JIEGUOYUFENXI

2.1不同培养基对猕猴桃花粉教你如何在网上轻松赚钱萌发和花粉管生长的影响

YANJIUBIAOMING,MIHOUTAOHUAFENZAIBUTONGPEIYANGJISHANGDEMENGFALVHEHUAFENGUANSHENGZHANGZHANGDUBIAOXIANBUTONG(BIAO1)。ZAI13、14、15、16SIZHONGPEIYANGJISHANGJIHUBUMENGFA,ZAIDI5、6、7、9、10、PEIYANGJISHANGJUNNENGJIAOHAODEMENGFA,MENGFALVDADAO50%YISHANG,QIZHONGZAIDI6ZHONGPEIYANGJISHANGMENGFALVZUIGAO,DADAOLE78.2%,YUQITAJIZHONGCHULICHAYIXIANZHU;CONGHUAFENGUANSHENGZHANGZHANGDULAIKAN,YESHIZAIDI6ZHONGPEIYANGJISHANGSHENGZHANGZUIZHANG,DADAO159.8um,YUQITAJIZHONGCHULICHAYIXIANZHU。YINCIRENWEI,DI6ZHONGPEIYANGJIZUISHIYIYUMIHOUTAOHUAFENDEMENGFAHEHUAFENGUANSHENGZHANGYANJIU,QIZUFENWEI: 10%TANG+100mg/LPENGSUAN+10mg/LCa(NO3) 2·4H2O。

培养环境条件对猕猴桃花粉萌发的影响 猕猴桃花粉萌发对授粉受精起着关键作用

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2.3PEIYANGSHIJIANDUIHUAFENMENGFAHEHUAFENGUANSHENGZHANGDEYINGXIANG

教你如何在网上轻松赚钱TU3、TU4FENBIEDAIBIAOBUTONGPEIYANGSHIJIANCHULIDUI‘ZHENGXIONG1HAO’HE‘ZHENGXIONG2HAO’LIANGGEPINZHONGHUAFENMENGFALVHEHUAFENGUANSHENGZHANGDEYINGXIANG。

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3.TAOLUN

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培养环境条件对猕猴桃花粉萌发的影响 猕猴桃花粉萌发对授粉受精起着关键作用

培养环境条件对猕猴桃花粉萌发的影响 猕猴桃花粉萌发对授粉受精起着关键作用猕猴桃花粉

培养环境条件对猕猴桃花粉萌发的影响 猕猴桃花粉萌发对授粉受精起着关键作用

培养环境条件对猕猴桃花粉萌发的影响 猕猴桃花粉萌发对授粉受精起着关键作用

培养环境条件对猕猴桃花粉萌发的影响 猕猴桃花粉萌发对授粉受精起着关键作用猕猴桃花粉授粉

培养环境条件对猕猴桃花粉萌发的影响 猕猴桃花粉萌发对授粉受精起着关键作用猕猴桃花粉检测

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教你如何在网上轻松赚钱